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mir30 cassette  (Addgene inc)


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    Structured Review

    Addgene inc mir30 cassette
    Mir30 Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mir30 cassette/product/Addgene inc
    Average 92 stars, based on 8 article reviews
    mir30 cassette - by Bioz Stars, 2026-02
    92/100 stars

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    (A) To create the Scn5a mice, a flox-stop-flox cassette from pCALSL-mir30 was inserted upstream of the human Scn5a-GFP cDNA, and this construct was targeted to the Rosa26 locus by electroporating into JM8.N4 C57BL/6N-derived ES cells. Two successfully targeted ES clones were injected into blastocysts and both lines were transmitted in the germline. (B) One line was selected to be bred to a CD4-Cre expressing strain, to yield Scn5a+CD4-Cre+ offspring.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Tuning T Cell Signaling Sensitivity Alters the Behavior of CD4+ T Cells During an Immune Response

    doi: 10.4049/jimmunol.1701422

    Figure Lengend Snippet: (A) To create the Scn5a mice, a flox-stop-flox cassette from pCALSL-mir30 was inserted upstream of the human Scn5a-GFP cDNA, and this construct was targeted to the Rosa26 locus by electroporating into JM8.N4 C57BL/6N-derived ES cells. Two successfully targeted ES clones were injected into blastocysts and both lines were transmitted in the germline. (B) One line was selected to be bred to a CD4-Cre expressing strain, to yield Scn5a+CD4-Cre+ offspring.

    Article Snippet: To create the Scn5a mice, a flox-stop-flox cassette from pCALSL-mir30 (a gift of Connie Cepko, Addgene plasmid #13786 ( 32 )) was inserted upstream of the human Scn5a-GFP cDNA (Genecopoeia, {"type":"entrez-nucleotide","attrs":{"text":"NM_198056.2","term_id":"124518659","term_text":"NM_198056.2"}} NM_198056.2 ).

    Techniques: Construct, Derivative Assay, Clone Assay, Injection, Expressing